Inhibition of penicillinase by epigallocatechin gallate resulting in restoration of antibacterial activity of penicillin against penicillinase-producing Staphylococcus aureus.
نویسندگان
چکیده
The combination of epigallocatechin gallate (EGCg, a main constituent of tea catechins) with penicillin showed synergism against 21 clinical isolates of penicillinase-producing Staphylococcus aureus. Besides binding directly to peptidoglycan, the inhibition of penicillinase activity by EGCg is responsible for the synergism. EGCg inhibited the penicillinase activity in a dose-dependent fashion, with a 50% inhibitory concentration of 10 microg/ml.
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The broth microdilution checkerboard and time-kill techniques were used to investigate the combination effects of (-)-epigallocatechin gallate (EGCg) and p-Iactams against various p-Iactamase-producing clinical isolates. Penicillin in combination with 12.5 ~g/ml EGCg showed the potent synergy against the penicillinase-producing S. aureus (21 strains) and restored the antibacterial activity of p...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 46 7 شماره
صفحات -
تاریخ انتشار 2002